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  • De Novo Sequencing
    • Overview
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De Novo Sequencing

현재 까지 밝혀지지 않은 미생물이나 동.식물의 전체 genome sequence를 생산하여 Genome Assembly 및 Annotation 결과를 바탕으로 육종이나 질병에 대한 유전적 요소들을 밝힐 수 있습니다.
신규유전체 해독에 적합한 Long reads 시퀀서로 Pac Bio System, Oxford Nanopore System을 보유 하고 있으며 조립결과를 개선 할 수 있는 10X Genomics및 Dovetail Hi-C 등의 최신 기술을 접목하여 월등히 향상된 결과를 제공 해 드립니다.

PacBio

Workflow
Sample requirements

10µg HMW DNA(>40Kb)

Turnaround time

About 4 weeks

10X Chromium

Workflow
Sample requirements

200ng HMW DNA(50~100Kb)

Turnaround time

About 4 weeks

Oxford Nanopore

Workflow
Sample requirements

5µg HMW DNA

Turnaround time

About 2 weeks

Bioinformatics

PacBio Oxford Nanopore Technology 10X Genomics Illumina
- Long-read Based Quality Genome Assembly Analysis
- Available Various Genome Size (over 20Gb
- Long-read Based Genome Assembly Analysis
- Linked-Read Based Genome Assembly Analysis
- Available Ultra Low Input DNA
- Short-read Based Genome Assembly Analysis
Dovetail Genomics Additional Data Report
- Chicago/Hi-C Hybrid Scaffolding Analysis - Hybrid Scaffolding/Assembly Analysis with Multiple Platform Data
- Phasing Haplotigs Analysis
- Gene Prediction with RNAseq, IsoSeq
- Gene Annotation with Various Databases (NCBI, Uniprot, GO, KEGG, InterProScan
- Sequencing Summary
- De novo Assembly Summary
- Assembly Assessment Summary
- Gene Prediction and Annotation (Excel, GFF, transcript/protein FASTA)
About
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