About
  • Our story
  • Where we began
  • Recent progress
  • Our Global Community
  • Leadership
  • Teams
Platform
  • AccuID
    • About AccuID
    • Document
    • AccuID FAQ
  • PDX
    • PDX Services
    • PDX Models
    • PDX Model browser
  • DNAGPS
Service
  • NGS
    • Overview
    • Whole Genome Resequencing
    • De Novo Sequencing
    • Whole exome/Targeted sequencing
    • Transcriptome Sequencing
    • Epigenome Sequencing
    • HLA Typing
    • Single cell sequencing
  • Microarray
    • DNA Analysis (GWAS, DMET)
    • RNA Analysis (mRNA, miRNA)
    • aCGH Analysis
    • CNV Analysis
  • Genotyping
    • Overview
    • Fluidigm BioMark system
    • TaqMan assay
    • SNaPshot assay
  • Digital ELISA Assay
Covid 19
Blog
Investors
Contact Us
NOTICE
DNA LINK
  • About
    • Our story
    • Where we began
    • Recent progress
    • Our Global Community
    • Leadership
    • Teams
  • Platform
    • AccuID
    • PDX
    • DNAGPS
  • Service
    • NGS
    • Microarray
    • Genotyping
    • Digital ELISA Assay
  • Covid 19
  • Blog
  • Investors
  • Contact Us
  • NOTICE
  • Home
  • Service
  • Microarray
    • NGS
    • Microarray
    • Genotyping
    • Digital ELISA Assay
  • RNA Analysis
    • DNA Analysis
    • RNA Analysis
    • aCGH Analysis
    • CNV Analysis

RNA Analysis(mRNA, miRNA)

서로 다른 condition에서 RNA의 상대적 양변화를 측정하여 전체 genome 상의 모든 유전자 발현 정도를 동시에 확인할 수 있는 방법입니다. 특정 condition, chemical, 조직 등에서 발현이 증가 또는 감소되는 유전자를 선별해 낼 수 있습니다.
Affymetirx array는 3' IVT expression analysis, Whole-Transcript(WT) expression analysis, miRNA analysis로 구분됩니다. Bioanalyzer 2100, Nanodrop 1000을 이용한 철저한 quality control 과정 을 통해 신뢰도 높은 결과를 제공합니다.

Example for workflow & requirement

Product

Brand
Array type
Type
Array
Min. Conc. & Amount
Used Amt for Array Analysis
Affymetrix 3'IVT
Expression
Eukaryotic
organism
Human U133+, Primeview, Mouse 430, Rat 230, Arabidopsis, Barley, Bovine, C. elegans, Canine, Chicken, Citrus, Cotton, Drosophila, E. coli, Maize, Medicago, P. aeruginosa, Plasmodium/Anopheles, Poplar, Porcine, Rhesus Macaque, Rice, S.aureus, Soybean, Sugar Cane, Tomato, Vitis vinifera (Grape), Wheat, Xenopus laevis, Xenopus tropicalis, Yeast, Zebrafish  130ng/ul, 10ul 390ng
Prokaryotic
organism
650ng/ul, 25ul 11ug
Whole-Transcript(WT) expression Human, Mouse, Rat, Human Exon, Mouse Exon, Rat Exon, Arabidopsis, Bovine, Canine, C. elegans, Chicken, Cyno-Rhes, Cynomolgus, Drosophila, Equine, Feline, Marmoset, Medicago, Ovine, Porcine, Rabbit, Rhesus, Rice (Cn), Rice (Jp), Rice (US), Soy Bean, Tomato, Zebra Finch, Zebrafish 100ng/ul, 10ul 300ng
miRNA(FlashTaq) Expression miRNA 3.0, miRNA 4.0 200ng/ul, 15ul 1.6ug
Agilent gene Expression Arabidopsis (V4), Barley, Bovine (V2), Brassica, Canine (V2), C. elegans (V2), Chicken (V2), Cotton, Drosophila, E. coli, Horse, Magnaporthe (V2), Medicago, Mosquito, Porcine (V2), Rhesus Macaque (V2), Rabbit, Rice, Salmon, Sheep, Tobacco, Tomato, Wheat, Xenopus (V2), Yeast (V2), Zebrafish (V3) 100ng/ul, 10ul  300ng
miRNA Human, Mouse, Rat    
           
Benefit

- 유전자, 전사체 및 miRNA의 최신의 data까지 포함하고 있어 포괄적인 연구가 가능함
- 다양한 포맷의 고객 맞춤형 content를 제공하므로 유연한 실험 디자인이 가능함
- Affymetirx array의 경우 cartridge 형태로 샘플 수량에 상관없이 원하는 개수로 분석 가능

Workflow

- biotin labeled cRNA 합성
- cRNA fragmentation
- expression array hybridization
- * Genechip Fluidics Station 450을 이용한 washing, staining
- Scanning through Genechip Array scanner 3000 7G
- Data Analysis

Sample requirement

RNA purity - 260/280 :1.8~2.1, 260/230 :1.8~2.1
RNA integrity - rRNA [28s / 18s] Ratio of over 1.5 , RIN value of over 8

Turnaround time
샘플 QC 후 3주일 이내( 샘플 수와 array 종류에 따라 변경 가능 ) 분석 결과 제공

3' IVT Expression Analysis

Build on the knowledge of thousands of publications with 3' IVT solutions–the industry-standard tool for gene expression research.

Cartridges
Human
  • Human Genome Focus Array
  • Human Genome U133 Plus 2.0 Array
  • Human Genome U133A 2.0 Array
  • Human Genome U133 Set
  • Human Genome U95 Set
  • PrimeView™ Human Gene Expression Array
  • Almac Xcel™ Array
Mouse
  • Mouse Expression Set 430
  • Mouse Genome 430 2.0 Array
  • Mouse Genome 430A 2.0 Array
  • Murine Genome U74v2 Set
Rat
  • Rat Expression Set 230
  • Rat Genome 230 2.0 Array
  • Rat Genome U34 Set
  • Rat ToxFX 1.0 Array
  • Rat Toxicology U34 Array
Plates
Human
  • HT HG-U133+ PM Array Plate
  • HT Human Genome U133 Array Plate Set
  • Human Genome U219 Array Plate
Mouse
  • HT MG-430 PM Array Plate
Rat
  • HT RG-230 PM Array Plate
Strips
Human
  • Human Genome U133 Array Strip
  • Human Genome U219 Array Strip
Mouse
  • MG-430 PM Array Strip
Rat
  • RG-230 PM Array Strip
CHO
  • CHO Gene ST Array Strip

Gene Regulation Analysis

GeneChip® Tiling Arrays are the most comprehensive array tools available for discovering novel RNA transcripts or mapping sites of protein/DNA interaction in chromatin immunoprecipitation experiments.

Cartridges
E. coli
  • Minimal Signature E coli Array0 Strip
Human
  • Chromosome 21 22 2.0R Array
  • Human Promoter 1.0R Array
  • Human Tiling 1.0R Array Set
  • Human Tiling 2.0R Array Setv
Mouse
  • Mouse Promoter 1.0R Array
  • Mouse Tiling 1.1R Array Set
  • Mouse Tiling 2.0R Array Set

miRNA Analysis

Affymetrix' miRNA Arrays offer the most comprehensive coverage of miRNAs on a single array. Combined with Affymetrix® Expression Console™ Software and FlashTag™ Labeling Kits, the complete miRNA solution is a powerful tool for exploring the role of miRNAs.

Cartridges
Human, Mouse, Rat, Other
  • miRNA Array
Plates
Human, Mouse, Rat, Other
  • miRNA Array Strip
Strips
Human, Mouse, Rat, Other
  • miRNA Array Plates

Whole-Transcript Expression Analysis & Profiling

Get the most complete view of the genome with our line of whole-transcript expression products.

Cartridges
Human
  • Human Transcriptome Array .0
  • Human Exon ST Array
  • Human Gene ST Arrays
Mouse
  • Mouse Exon ST Array
  • Mouse Gene ST Arrays
Rat
  • Rat Exon ST Array
  • Rat Gene ST Arrays
CHO
  • CHO Gene ST Arrays
Model and applied research organisms
  • Model and applied research organisms Gene 1.0 ST Array
Plates
Human
  • Human Gene ST Array Plates
Mouse
  • Mouse Gene ST Array Plates
Rat
  • Rat Gene ST Array Plates
CHO
  • CHO Gene ST Array Plates
Model and applied research organisms Gene 1.1 Array Plates
  • Model and applied research organisms Gene 1.1 ST Array Plates
Strips
Human
  • Human Gene ST Array Strips
Mouse
  • Mouse Gene ST Array Strips
Rat
  • Rat Gene ST Array Strips
CHO
  • CHO Gene ST Array Strip
Model and applied research organisms Gene 1.1 Array Plates
  • Model and applied research organisms Gene 1.1 ST Array Strips

Bioinformatics

SNP Array
- Gender,Call rate, Hetero rate, QC, IBS test Summary
- GWAS(Genome-Wide Association Study) Analysis
- AberrationSummary
- DetectionAmplification, Deletion and LOH
About
  • Our story
  • Where we began
  • Recent progress
  • Our Global Community
  • Leadership
  • Teams
Platform
  • AccuID
  • PDX
  • DNAGPS
Service
  • NGS
  • Microarray
  • Genotyping
  • Digital ELISA Assay
Covid 19
Blog
Investors
Contact Us
Notice
© DNALINK - All Rights Reserved.