서로 다른 condition에서 RNA의 상대적 양변화를 측정하여 전체 genome 상의 모든 유전자 발현 정도를 동시에 확인할 수 있는 방법입니다. 특정 condition, chemical, 조직 등에서 발현이 증가 또는 감소되는 유전자를 선별해 낼 수 있습니다.
Affymetirx array는 3' IVT expression analysis, Whole-Transcript(WT) expression analysis, miRNA analysis로 구분됩니다. Bioanalyzer 2100, Nanodrop 1000을 이용한 철저한 quality control 과정 을 통해 신뢰도 높은 결과를 제공합니다.
| Brand |
Array type |
Type |
Array |
Min. Conc. & Amount |
Used Amt for Array Analysis |
| Affymetrix | 3'IVT Expression |
Eukaryotic organism |
Human U133+, Primeview, Mouse 430, Rat 230, Arabidopsis, Barley, Bovine, C. elegans, Canine, Chicken, Citrus, Cotton, Drosophila, E. coli, Maize, Medicago, P. aeruginosa, Plasmodium/Anopheles, Poplar, Porcine, Rhesus Macaque, Rice, S.aureus, Soybean, Sugar Cane, Tomato, Vitis vinifera (Grape), Wheat, Xenopus laevis, Xenopus tropicalis, Yeast, Zebrafish | 130ng/ul, 10ul | 390ng |
| Prokaryotic organism |
650ng/ul, 25ul | 11ug | |||
| Whole-Transcript(WT) expression | Human, Mouse, Rat, Human Exon, Mouse Exon, Rat Exon, Arabidopsis, Bovine, Canine, C. elegans, Chicken, Cyno-Rhes, Cynomolgus, Drosophila, Equine, Feline, Marmoset, Medicago, Ovine, Porcine, Rabbit, Rhesus, Rice (Cn), Rice (Jp), Rice (US), Soy Bean, Tomato, Zebra Finch, Zebrafish | 100ng/ul, 10ul | 300ng | ||
| miRNA(FlashTaq) Expression | miRNA 3.0, miRNA 4.0 | 200ng/ul, 15ul | 1.6ug | ||
| Agilent | gene Expression | Arabidopsis (V4), Barley, Bovine (V2), Brassica, Canine (V2), C. elegans (V2), Chicken (V2), Cotton, Drosophila, E. coli, Horse, Magnaporthe (V2), Medicago, Mosquito, Porcine (V2), Rhesus Macaque (V2), Rabbit, Rice, Salmon, Sheep, Tobacco, Tomato, Wheat, Xenopus (V2), Yeast (V2), Zebrafish (V3) | 100ng/ul, 10ul | 300ng | |
| miRNA | Human, Mouse, Rat | ||||
- 유전자, 전사체 및 miRNA의 최신의 data까지 포함하고 있어 포괄적인 연구가 가능함
- 다양한 포맷의 고객 맞춤형 content를 제공하므로 유연한 실험 디자인이 가능함
- Affymetirx array의 경우 cartridge 형태로 샘플 수량에 상관없이 원하는 개수로 분석 가능
- biotin labeled cRNA 합성
- cRNA fragmentation
- expression array hybridization
- * Genechip Fluidics Station 450을 이용한 washing, staining
- Scanning through Genechip Array scanner 3000 7G
- Data Analysis
RNA purity - 260/280 :1.8~2.1, 260/230 :1.8~2.1
RNA integrity - rRNA [28s / 18s] Ratio of over 1.5 , RIN value of over 8
Build on the knowledge of thousands of publications with 3' IVT solutions–the industry-standard tool for gene expression research.
GeneChip® Tiling Arrays are the most comprehensive array tools available for discovering novel RNA transcripts or mapping sites of protein/DNA interaction in chromatin immunoprecipitation experiments.
Affymetrix' miRNA Arrays offer the most comprehensive coverage of miRNAs on a single array. Combined with Affymetrix® Expression Console™ Software and FlashTag™ Labeling Kits, the complete miRNA solution is a powerful tool for exploring the role of miRNAs.
Get the most complete view of the genome with our line of whole-transcript expression products.
| SNP Array |
| - Gender,Call rate, Hetero rate, QC, IBS test Summary - GWAS(Genome-Wide Association Study) Analysis - AberrationSummary - DetectionAmplification, Deletion and LOH |